Himedia 500g Bacteriological Grade Agar Powder

Agar powder, Bacteriological grade

Agar powder, Bacteriological grade is recommended for routine bacteriological work

Cooling temperature not more than 40°C
Recommended for routine bacteriological work where compatibility with other ingredients is not a critical criteria
NOTE: Use for Plant Tissue Culture Media.
It is recommended to adjust the desired pH of the medium after the addition of Agar

Quality Control
Appearance : Cream coloured powder homogeneous free flowing powder
Solubility : Freely soluble in hot water at temperatures above 85°C. Insoluble in cold water.
Clarity : A firm solid, clear to slightly opalescent gel is formed at a concentration of 1.5% at 38-40°C.
Dye Diffusion : Agar dye diffusion :- 18-20mm
pH : pH of 1.5% w/v aqueous solution at 25 °C 6.5 - 7.5
Identification test : As per method specified in USP 2022
A: Infrared absorption
B: Iodine TS colours some of the fragments of the Agar bluish black, with some areas reddish to violet.
C: Agar forms a clear liquid that congeals at 30-39 ° C to form a firm resilient gel, which does not liquefy below
80°C.
Microbial Load :
Bacterial Count : <= 1000 CFU/gram by plate method, when incubated at 30-35ºC for not less than 3 days
Yeast & mould Count : <= 100 CFU/gram by plate method, when incubated at 20-25ºC for not less than 5 days.
Test for pathogens : 1. Escherichia coli- Absent/gram of sample 2. Salmonella species- Absent/10 gram of sample
3. Pseudomonas aeruginosa- Absent/gram of sample 4. Staphylococcus aureus- Absent/gram of sample 5. Candida
albicans- Absent/gram of sample 6. Clostridia- Absent/gram of samp

Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at the recommended temperature