Himedia 500g Granulated Lactobacillus MRS Agar

GM641

Lactobacillus MRS Agar, Granulated (MRS Agar, Granulated) is recommended for isolation and cultivation of Lactobacilli.

Proteose peptone, HM Ppetone B, yeast extract , dextrose provides carbon and nitrogen sources for lactobacilli growth.

Sodium acetate and ammonium citrate inhibit Streptococci, moulds and many other microorganisms. Magnesium sulphate and manganese sulphate provide essential ions for multiplication of lactobacilli. Phosphates provide good buffering action in the media.

Polysorbate 80 supplies fatty acids required for the metabolism of Lactobacilli.

Quality Control
Appearance-Cream to light yellow coloured granular medium.
Gelling-Firm, comparable with 1.2% Agar gel.
Colour and Clarity of prepared medium
Medium to dark amber coloured slightly opalescent gel forms in Petri plates
Reaction
Reaction of 6.71% w/v aqueous solution at 25°C. pH :6.5±0.2
pH
6.30-6.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours or longer.(with 5% CO2)

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